Name the technique used to separate leaf pigments.
GeneralClass 12AllAnswered 27 Mar 2026
Answer
Paper chromatography (or thin-layer chromatography) separates photosynthetic pigments based on their differential solubility in organic solvents and adsorption rates on paper/silica gel.
Typical Rf values (in petroleum ether:acetone):
- Carotene: 0.95 (highest, most non-polar)
- Chlorophyll a: 0.65
- Chlorophyll b: 0.45
- Xanthophylls: 0.35 (lowest, most polar)
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