Explain T5 exonuclease activity in simple short points

T5 exonuclease is an enzyme that digests DNA from its ends and produces dNMPs and short oligonucleotides.

The diagram shows T5 exonuclease breaking DNA from the ends and producing dNMPs and short oligos.

T5 Exonuclease Activity:

• Starting material – different DNA end structures used as substrates
• DNA – double-stranded DNA molecule
• 5′ end – starting direction marker of DNA strand
• 3′ end – opposite direction marker of DNA strand
• T5 exonuclease – enzyme that digests DNA from the ends
• dNMP – single deoxynucleotide monophosphate released after digestion
• Short oligos – short DNA fragments produced after enzyme action