Explain pBR322 plasmid vector

pBR322 is a cloning vector with ori, ampicillin resistance, tetracycline resistance, and several restriction sites.

The diagram shows the pBR322 plasmid, a circular DNA cloning vector used in genetic engineering.

• pBR322 – plasmid vector of 4361 base pairs
• ori – origin of replication; helps plasmid copy itself
• amp – ampicillin resistance gene
• tet – tetracycline resistance gene
• EcoRI – restriction enzyme site
• HindIII – restriction enzyme site
• EcoRV – restriction enzyme site
• BamHI – restriction enzyme site
• SalI – restriction enzyme site
• NdeI – restriction enzyme site
• PstI – restriction enzyme site