Explain EcoRI restriction enzyme and recombinant DNA formation

EcoRI cuts DNA at GAATTC, producing sticky ends that join to form recombinant DNA.

The diagram shows how the restriction enzyme EcoRI cuts vector DNA and foreign DNA at the sequence GAATTC, forming sticky ends that join to make recombinant DNA.

• Vector DNA – DNA used to carry foreign DNA
• Foreign DNA – DNA segment to be inserted
• EcoRI – restriction enzyme that cuts DNA
• GAATTC – recognition sequence for EcoRI
• Sticky end – single-stranded overhang formed after cutting
• DNA fragments join at sticky ends – matching sticky ends pair together
• Recombinant DNA – DNA formed by joining vector DNA and foreign DNA